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Efficient purification of cell culture-derived classical swine fever virus by ultrafiltration and size-exclusion

Ruining WANG,Yubao ZHI,Junqing GUO,Qingmei LI,Li WANG,Jifei YANG,Qianyue JIN,Yinbiao WANG,Yanyan YANG,Guangxu XING,Songlin QIAO,Mengmeng ZHAO,Ruiguang DENG,Gaiping ZHANG

《农业科学与工程前沿（英文）》 2015年 第2卷 第3期   页码 230-236 doi: 10.15302/J-FASE-2015071

摘要： Large-scale production of cell culture-based classical swine fever virus (CSFV) vaccine is hampered by the adverse reactions caused by contaminants from host cell and culture medium. Hence, we have developed an efficient method for purifying CSFV from cell-culture medium. Pure viral particles were obtained with two steps of tangential-flow filtration (TFF) and size-exclusion chromatography (SEC), and were compared with particles from ultracentrifugation by transmission electron microscopy (TEM), infectivity and recovery test, and real time fluorescent quantitative PCR (FQ-PCR). TFF concentrated the virus particles effectively with a retention rate of 98.5%, and 86.2% of viral particles were obtained from the ultrafiltration retentate through a Sepharose 4 F F column on a biological liquid chromatography system. CSFV purified by TFF-SEC or ultracentrifugation were both biologically active from 1.0×10 TCID ·mL to 3.0×10 TCID ·mL , but the combination of TFF and SEC produced more pure virus particles than by ultracentrifugation alone. In addition, pure CSFV particles with the expected diameter of 40–60 nm were roughly spherical without any visible contamination. Mice immunized with CSFV purified by TFF-SEC produced higher antibody levels compared with immunization with ultracentrifugation-purified CSFV ( <0.05). The purification procedures in this study are reliable technically and feasible for purification of large volumes of viruses.

关键词： classical swine fever virus     virus purification     tangential-flow filtration     size-exclusion chromatography    

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Yellow fever and Hajj: with all eyes on Zika, a familiar flavivirus remains a threat

null

《医学前沿（英文）》 2016年 第10卷 第4期   页码 527-530 doi: 10.1007/s11684-016-0487-2

摘要：

Hajj is among the world’s largest mass gatherings, drawing between 2 and 3.5 million Muslims from 183 nations annually to perform pilgrimage in Mecca, Saudi Arabia. Infectious disease outbreaks can be imported both into the Hajj population and exported internationally by returning pilgrims. The domestic Saudi population can also be at risk of outbreaks traveling amid this mass migration. With yellow fever reported for the first time in China following the infection of expatriate Chinese workers in Angola and a full blown outbreak underway in wider West Africa, the prospect of yellow fever outbreaks in Asia threatens to impact Saudi Arabia, both during and beyond the Hajj season. With global focus trained on Zika, the rising threat of yellow fever cannot be overlooked. Strategies to mitigate risk to Saudi Arabia and the global population are thereby suggested.

关键词： yellow fever     mass gathering     Saudi Arabia     Hajj     Zika virus    

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Soundscape of classical Chinese garden

YUAN Xiaomei, WU Shuoxian

《结构与土木工程前沿（英文）》 2008年 第2卷 第2期   页码 172-178 doi: 10.1007/s11709-008-0026-6

摘要： With deep humanized connotation, the classical Chinese garden uses human intuitive sensation and personal poetic observation to express natural sound phenomena. It differs from the rational modern soundscape in western countries.

关键词： observation     classical     phenomena     intuitive     soundscape    

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Field scale measurement of greenhouse gas emissions from land applied swine manure

Devin L. Maurer, Jacek A. Koziel, Kelsey Bruning

《环境科学与工程前沿（英文）》 2017年 第11卷 第3期 doi: 10.1007/s11783-017-0915-9

摘要： Greenhouse gas emissions (GHGs) from swine production systems are relatively well researched with the exception of emissions from land application of manure. GHGs inventories are needed for process-based modeling and science-based regulations. Thus, the objective of this observational study was to measure GHG fluxes from land application of swine manure on a typical corn field. Assessment of GHG emissions from deep injected land-applied swine manure, fall and reapplication in the spring, on a typical US Midwestern corn-on-corn farm was completed. Static chambers were used for flux measurement along with gas analysis on a GC-FID-ECD. Measured gas concentrations were used to estimate GHGs flux using four different models: linear regression, nonlinear regression, first order linear regression and the revised Hutchinson and Mosier (HMR) model, respectively for comparisons. Cumulative flux estimates after manure application of 5.85 × 10 g·ha (1 ha= 0.01 km ) of CO , 6.60 × 10 g·ha of CH , and 3.48 × 10 g·ha N O for the fall trial and 3.11 × 10 g·ha of CO , 2.95 × 10 g·ha of CH , and 1.47 × 10 g·ha N O after the spring reapplication trial were observed. The N O net cumulative flux represents 0.595% of nitrogen applied in swine manure for the fall trial.

关键词： Climate change     Emissions     Greenhouse gases     Land application     Swine manure    

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Occurrence of veterinary antibiotics in struvite recovery from swine wastewater by using a fluidized

Zhi-Long Ye, Yujun Deng, Yaoyin Lou, Xin Ye, Shaohua Chen

《环境科学与工程前沿（英文）》 2018年 第12卷 第3期 doi: 10.1007/s11783-018-1015-1

摘要： Recovering phosphorus from livestock wastewater has gained extensive attention. The residue of veterinary antibiotics in the wastewater may be present in the recovered products, thereby posing pharmacological threats to the agricultural planting and human health. This study investigated antibiotic occurrence in the struvite particles recovered from swine wastewater by using a fluidized bed. Results revealed that tetracyclines possessed significant residues in the struvite granules, with the values ranging from 195.2 mg·kg to 1995.0 mg·kg . As for fluoroquinolones, their concentrations varied from 0.4 mg·kg to 1104.0 mg·kg . Struvite particles were of various sizes and shapes and displayed different antibiotic adsorption capacities. The data also showed that the smaller granules contained much more antibiotics than the larger ones, indicating that the fluidized granulation process of struvite crystals plays an important role on the accumulation of antibiotics. For tetracyclines, organic matters and struvite adsorption exerted significant impacts on tetracyclines migration. The outcomes underscore the need to consider the residues of antibiotics in resource recovery from wastewater because they exert pharmacological impacts on the utilization of recovered products.

关键词： Antibiotic     Struvite     Phosphorus recovery     Swine wastewater     Fluidized bed    

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and microbial analysis in partially saturated constructed wetland for treating anaerobically digested swine

Zhenfeng Han, Ying Miao, Jing Dong, Zhiqiang Shen, Yuexi Zhou, Shan Liu, Chunping Yang

《环境科学与工程前沿（英文）》 2019年 第13卷 第4期 doi: 10.1007/s11783-019-1133-4

摘要：

Anaerobically digested swine wastewater was treated by a novel constructed wetland.

Tidal operation was better for total nitrogen removal than intermittent flow.

Mechanism of nitrogen removal by biozeolite-based constructed wetland was discussed.

Simultaneous nitrification and denitrification were determined in zeolite layer.

关键词： Constructed wetland     Decentralized swine wastewater     Biozeolite     Simultaneous nitrification and denitrification     Nitrogen removal pathway    

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Yue’s solution of classical elasticity in

Zhong-qi Quentin YUE

《结构与土木工程前沿（英文）》 2015年 第9卷 第3期   页码 215-249 doi: 10.1007/s11709-015-0298-6

摘要： This paper presents the exact and complete fundamental singular solutions for the boundary value problem of a -layered elastic solid of either transverse isotropy or isotropy subject to body force vector at the interior of the solid. The layer number is an arbitrary nonnegative integer. The mathematical theory of linear elasticity is one of the most classical field theories in mechanics and physics. It was developed and established by many well-known scientists and mathematicians over 200 years from 1638 to 1838. For more than 150 years from 1838 to present, one of the remaining key tasks in classical elasticity has been the mathematical derivation and formulation of exact solutions for various boundary value problems of interesting in science and engineering. However, exact solutions and/or fundamental singular solutions in closed form are still very limited in literature. The boundary-value problems of classical elasticity in -layered and graded solids are also one of the classical problems challenging many researchers. Since 1984, the author has analytically and rigorously examined the solutions of such classical problems using the classical mathematical tools such as Fourier integral transforms. In particular, he has derived the exact and complete fundamental singular solutions for elasticity of either isotropic or transversely isotropic layered solids subject to concentrated loadings. The solutions in -layered or graded solids can be calculated with any controlled accuracy in association with classical numerical integration techniques. Findings of this solution formulation are further used in the companion paper for mathematical verification of the solutions and further applications for exact and complete solutions of other problems in elasticity, elastodynamics, poroelasticty and thermoelasticity. The mathematical formulations and solutions have been named by other researchers as Yue’s approach, Yue’s treatment, Yue’s method and Yue’s solution.

关键词： elasticity     solution     layered solid     graded material    

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痩肉猪育种的发展及展望

熊远著

《中国工程科学》 2000年 第2卷 第9期   页码 42-46

摘要：

文章对世界的猪育种过程特别是中国的瘦肉猪育种，从猪种资源调査到优良猪种引进与新品种选育，专门化品系选育与配套利用，种猪测定等方面进行了回顾，就分子生物技术在猪遗传育种中的应用及取得的成果作了简要概述。展望了21世纪瘦肉猪育种的目标，并就新品种培育、猪种资源保存、完善种猪测定体系和加速分子生物技术在猪育种中的应用提出了建议。

关键词： 猪     瘦肉型     育种    

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metagenomic analysis reveals different functional potentials of multiple Brocadia species in a full-scale swine

《环境科学与工程前沿（英文）》 2023年 第17卷 第1期 doi: 10.1007/s11783-023-1602-7

摘要：

● Four Ca. Brocadia species were observed during the spontaneously enrichment.

关键词： Anammox     Candidatus Brocadia     Functional potential     Cooccurring mechanisms     Swine wastewater treatment facilities    

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Removal of virus aerosols by the combination of filtration and UV-C irradiation

《环境科学与工程前沿（英文）》 2023年 第17卷 第3期 doi: 10.1007/s11783-023-1627-y

摘要：

● The removal of virus aerosols by filtration and UV-C irradiation was proposed.

关键词： Filtration system     UV-C irradiation     Virus aerosol     Public health     COVID-19    

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Critical roles of chemokines and cytokines in antiviral innate immune responses during rabies virus infection

Ying HUANG, Clement Wesley GNANADURAI, Zhenfang FU

《农业科学与工程前沿（英文）》 2017年 第4卷 第3期   页码 260-267 doi: 10.15302/J-FASE-2016116

摘要： The innate immune response is the first line of defense against viral invasion and pro-inflammatory chemokines and cytokines have a critical function in the innate immune responses against virus infections. The ability of a rabies virus (RABV) to induce the expression of chemokines and cytokines can lead to viral clearance from the central nervous system (CNS), whereas the ability to evade such expression and activation contributes to virulence and pathogenicity. In this review, the crucial contribution of chemokines/cytokines to clearing RABV from the CNS is discussed, including recruiting leukocytes into the CNS, enhancement of blood brain barrier permeability and activation of various immune cells that are essential for viral clearance. In addition, recombinant RABV expressing cytokines and chemokines can induce elevated innate and adaptive immune responses which result in clearing an established wild-type RABV infection in the CNS.

关键词： antiviral     blood brain barrier     chemokines and cytokines     innate immunity     rabies virus    

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Immunological and virological characteristics of human immunodeficiency virus type 1 superinfection:

null

《医学前沿（英文）》 2017年 第11卷 第4期   页码 480-489 doi: 10.1007/s11684-017-0594-8

摘要：

Superinfection is frequently detected among individuals infected by human immunodeficiency virus type I (HIV-1). Superinfection occurs at similar frequencies at acute and chronic infection stages but less frequently than primary infection. This observation indicates that the immune responses elicited by natural HIV-1 infection may play a role in curb of superinfection; however, these responses are not sufficiently strong to completely prevent superinfection. Thus, a successful HIV-1 vaccine likely needs to induce more potent and broader immune responses than those elicited by primary infection. On the other hand, potent and broad neutralization responses are more often detected after superinfection than during monoinfection. This suggests that broadly neutralizing antibodies are more likely induced by sequential immunization of multiple different immunogens than with only one form of envelope glycoprotein immunogens. Understanding why the protection from superinfection by immunity induced by primary infection is insufficient and if superinfection can lead to cross-reactive immune responses will be highly informative for HIV-1 vaccine design.

关键词： human immunodeficiency virus type I     superinfection     incidence     immune response    

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Unit commitment using dynamic programming–an exhaustive working of both classical and stochastic approach

Balasubramaniyan SARAVANAN, Surbhi SIKRI, K. S. SWARUP, D. P. KOTHARI

《能源前沿（英文）》 2013年 第7卷 第3期   页码 333-341 doi: 10.1007/s11708-013-0259-5

摘要： In the present electricity market, where renewable energy power plants have been included in the power systems, there is a lot of unpredictability in the demand and generation. There are many conventional and evolutionary programming techniques used for solving the unit commitment (UC) problem. Dynamic programming (DP) is a conventional algorithm used to solve the deterministic problem. In this paper DP is used to solve the stochastic model of UC problem. The stochastic modeling for load and generation side has been formulated using an approximate state decision approach. The programs were developed in a MATLAB environment and were extensively tested for a four-unit eight-hour system. The results obtained from these techniques were validated with the available literature and outcome was good. The commitment is in such a way that the total cost is minimal. The novelty of this paper lies in the fact that DP is used for solving the stochastic UC problem.

关键词： unit commitment (UC)     deterministic     stochastic     dynamic programming (DP)     optimization     state diagram    

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Mesophilic and thermophilic anaerobic digestion of swine manure with sulfamethoxazole and norfloxacin

《环境科学与工程前沿（英文）》 2021年 第15卷 第5期 doi: 10.1007/s11783-020-1342-x

摘要：

• SMX addition had negative effect on acetoclastic methanogens in mesophilic AD.

关键词： Pig manure     Antibiotics     Anaerobic digestion     Resistance genes     Microbial community    

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Methane, Nitrous Oxide and Ammonia generation in full-scale swine wastewater purification facilities

Takashi Osada, Makoto Shiraishi, Teruaki Hasegawa, Hirofumi Kawahara

《环境科学与工程前沿（英文）》 2017年 第11卷 第3期 doi: 10.1007/s11783-017-0933-7

摘要： The activated sludge process to remove nitrogen and biochemical oxygen demand (BOD) is reportedly cost-effective for swine wastewater treatment, and it use has thus increased in pig farming. Nitrous oxide (N O) is generated on farms as an intermediate product in nitrification and denitrification, and methane (CH ) is also generated from organic degradation under anaerobic conditions by microorganisms in manure or wastewater. This study was carried out at five activated sludge treatment facilities across Japan between August 2014 and January 2015. Measurements were conducted over several weeks at wastewater purification facilities for swine farms: two in Chiba prefecture (East Japan), two in Okayama prefecture (West Japan), and one in Saga (Southern Japan). Taking several environmental fluctuations into account, we collected measurement data continuously day and night, during both high-temperature and low-temperature periods. The results indicated that CH and N O emission factors were 0.91% (kgCH · kg volatile solids ) and 2.87% (g N O-N· kg total N ), respectively. Ammonia emissions were negligible in all of the measurements from the wastewater facilities. The N O emission factor calculated under this experiment was low compared to our previous finding (5.0%; g N O-N· kg N ) in a laboratory experiment. In contrast, the CH emission factor calculated herein was rather high compared to the laboratory measurements. There was great variation in daily GHG emission factors measured in the actual wastewater treatment facilities. In particular, the N O emission rate was affected by several environmental conditions at each facility location, as well as by the management of the wastewater treatment.

关键词： Manure     Greenhouse gas     Denitrification     BOD/N     Nitrous oxide     Methane    

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标题 作者 时间 类型 操作

Efficient purification of cell culture-derived classical swine fever virus by ultrafiltration and size-exclusion

Ruining WANG,Yubao ZHI,Junqing GUO,Qingmei LI,Li WANG,Jifei YANG,Qianyue JIN,Yinbiao WANG,Yanyan YANG,Guangxu XING,Songlin QIAO,Mengmeng ZHAO,Ruiguang DENG,Gaiping ZHANG

期刊论文

Yellow fever and Hajj: with all eyes on Zika, a familiar flavivirus remains a threat

null

期刊论文

Soundscape of classical Chinese garden

YUAN Xiaomei, WU Shuoxian

期刊论文

Field scale measurement of greenhouse gas emissions from land applied swine manure

Devin L. Maurer, Jacek A. Koziel, Kelsey Bruning

期刊论文

Occurrence of veterinary antibiotics in struvite recovery from swine wastewater by using a fluidized

Zhi-Long Ye, Yujun Deng, Yaoyin Lou, Xin Ye, Shaohua Chen

期刊论文

and microbial analysis in partially saturated constructed wetland for treating anaerobically digested swine

Zhenfeng Han, Ying Miao, Jing Dong, Zhiqiang Shen, Yuexi Zhou, Shan Liu, Chunping Yang

期刊论文

Yue’s solution of classical elasticity in

Zhong-qi Quentin YUE

期刊论文

痩肉猪育种的发展及展望

熊远著

期刊论文

metagenomic analysis reveals different functional potentials of multiple Brocadia species in a full-scale swine

期刊论文

Removal of virus aerosols by the combination of filtration and UV-C irradiation

期刊论文

Critical roles of chemokines and cytokines in antiviral innate immune responses during rabies virus infection

Ying HUANG, Clement Wesley GNANADURAI, Zhenfang FU

期刊论文

Immunological and virological characteristics of human immunodeficiency virus type 1 superinfection:

null

期刊论文

Unit commitment using dynamic programming–an exhaustive working of both classical and stochastic approach

Balasubramaniyan SARAVANAN, Surbhi SIKRI, K. S. SWARUP, D. P. KOTHARI

期刊论文

Mesophilic and thermophilic anaerobic digestion of swine manure with sulfamethoxazole and norfloxacin

期刊论文

Methane, Nitrous Oxide and Ammonia generation in full-scale swine wastewater purification facilities

Takashi Osada, Makoto Shiraishi, Teruaki Hasegawa, Hirofumi Kawahara

期刊论文